Comparison of nine media in the culture of human ovarian granulosa lutein cells.

BACKGROUND Cultures of human ovarian granulosa lutein (hGL) cells are broadly used in experimental studies. The choice of the culture medium is important for the optimization of the conditions for culture of hGL cells. AIM To compare the efficiency of a basic salt solution and eight different defined media on the culture of hGL cells. MATERIALS AND METHODS Cultures of the HGL-5 cell line were maintained for 72 hours with DMEM/F12, RPMI-1640, Ham'sF10, Modified Ham'sF10®, HTFXtra®, Global®, Complete Multiblast®, Universal® or Earle's balanced salt solution (EBSS). At the end of the culturing period, the attachment, the viability and the total number of cells were measured. RESULTS Culture in DMEM/F12 led to the highest score of all studied parameters, followed by RPMI-1640. The lowest performance was recorded with Complete Multiblast® and EBSS. The use of the other media gave mediocre results. CONCLUSION Among the media tested, DMEM/F12 appears to be the best choice for the culture of hGL cells.